Hotspot

trnfM-CAU-rbcL

Genome · 106304-106880 · 577 bp

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Pi 0.136

MarkerSeek score 83.40

Primer available yes

Label trnfM-CAU-rbcL

Feature Metadata

label_name: trnfM-CAU-rbcL
feature_type: igs
region: Genome
length_bp: 577
variable_sites: 72
pi: 0.136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rbcL_p1	AACCTTGAAGACACGGGTTC	CCAATCGGGTCGCTTTAGAA	50.0	50.0	58.0	58.0	787-871 bp	1.0	91.0
trnfM-CAU-rbcL_p2	AACCTTGAAGACACGGGTTC	ACGGGTGAGATTAAGGGACA	50.0	50.0	58.0	58.1	1349-1433 bp	1.0	86.7
trnfM-CAU-rbcL_p3	CCTTGAAGACACGGGTTCAA	CCAATCGGGTCGCTTTAGAA	50.0	50.0	58.0	58.0	785 bp	0.5	59.5
trnfM-CAU-rbcL_p4	AACCCCATTGCCGATTTCTT	CCAATCGGGTCGCTTTAGAA	45.0	50.0	58.1	58.0	1383 bp	0.5	59.3
trnfM-CAU-rbcL_p5	ACCCCATTGCCGATTTCTTT	CCAATCGGGTCGCTTTAGAA	45.0	50.0	58.1	58.0	1382 bp	0.5	59.3