Hotspot

ndhC-chlN

Genome · 72923-74068 · 1146 bp

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Pi 0.5

MarkerSeek score 81.50

Primer available yes

Label ndhC-chlN

Feature Metadata

label_name: ndhC-chlN
feature_type: igs
region: Genome
length_bp: 1146
variable_sites: 501
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-chlN_p1	TTAAGACCATTCCAGCGCTC	GCTCCTTTTCCTATCGGTCC	50.0	55.0	58.0	57.8	2082 bp	0.5	57.9
ndhC-chlN_p2	TTAAGACCATTCCAGCGCTC	TGAAGGAAAGGGAGGAACGA	50.0	50.0	58.0	58.3	2000 bp	0.5	57.6
ndhC-chlN_p3	TTAAGACCATTCCAGCGCTC	TAGCTATCACGGGAATGGCT	50.0	50.0	58.0	58.3	1700 bp	0.5	57.5
ndhC-chlN_p4	GACTGGATCTTGGCACCAAA	GCTCCTTTTCCTATCGGTCC	50.0	55.0	58.1	57.8	2346 bp	0.5	57.3
ndhC-chlN_p5	GTCACAGTTCCGGCTGTTAT	GCTCCTTTTCCTATCGGTCC	50.0	55.0	57.9	57.8	2386 bp	0.5	57.3