Hotspot

trnE-UUC-psaM

Genome · 69974-70414 · 441 bp

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Pi 0.478

MarkerSeek score 72.80

Primer available yes

Label trnE-UUC-psaM

Feature Metadata

label_name: trnE-UUC-psaM
feature_type: igs
region: Genome
length_bp: 441
variable_sites: 144
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psaM_p1	TTGACAGGGCGGTACTCTAA	ACAAACAAAGCCTCAAAGCG	50.0	45.0	58.1	57.8	1113 bp	0.5	57.8
trnE-UUC-psaM_p2	TAGTCATTCAACTGTGCCGG	ACAAACAAAGCCTCAAAGCG	50.0	45.0	57.9	57.8	1052 bp	0.5	57.6
trnE-UUC-psaM_p3	TGGATTTGAACCAGCGTAGG	ACAAACAAAGCCTCAAAGCG	50.0	45.0	57.9	57.8	951 bp	0.5	57.5
trnE-UUC-psaM_p4	TTGACAGGGCGGTACTCTAA	TTTCTCTAGCCTGGCCAGTA	50.0	50.0	58.1	57.8	982 bp	0.5	57.4
trnE-UUC-psaM_p5	AGGGCGGTACTCTAACCAAT	ACAAACAAAGCCTCAAAGCG	50.0	45.0	58.1	57.8	1108 bp	0.5	57.3