Hotspot

psbD-trnD-GUC

Genome · 68363-69558 · 1196 bp

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Pi 0.503

MarkerSeek score 83.00

Primer available yes

Label psbD-trnD-GUC

Feature Metadata

label_name: psbD-trnD-GUC
feature_type: igs
region: Genome
length_bp: 1196
variable_sites: 558
pi: 0.503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnD-GUC_p1	AGCACATAGAAGAGCAGCAC	TTAGAGTACCGCCCTGTCAA	50.0	50.0	58.0	58.1	1890 bp	0.5	59.2
psbD-trnD-GUC_p2	AGCACATAGAAGAGCAGCAC	CCGGCACAGTTGAATGACTA	50.0	50.0	58.0	57.9	1951 bp	0.5	59.1
psbD-trnD-GUC_p3	CCAATACTCCAGCAACTCCC	TTAGAGTACCGCCCTGTCAA	55.0	50.0	58.0	58.1	1870 bp	0.5	59.1
psbD-trnD-GUC_p4	CCCAATACTCCAGCAACTCC	TTAGAGTACCGCCCTGTCAA	55.0	50.0	58.0	58.1	1871 bp	0.5	59.1
psbD-trnD-GUC_p5	TACTCCAGCAACTCCCATCA	TTAGAGTACCGCCCTGTCAA	50.0	50.0	58.0	58.1	1866 bp	0.5	59.0