Hotspot

ycf2-ndhB

Genome · 29209-29953 · 745 bp

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Pi 0.169

MarkerSeek score 83.60

Primer available yes

Label ycf2-ndhB

Feature Metadata

label_name: ycf2-ndhB
feature_type: igs
region: Genome
length_bp: 745
variable_sites: 118
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-ndhB_p1	GTCTTGTACAGGCGGCAATA	ATTCTGGTGTGGATGGCAAG	50.0	50.0	58.0	58.2	1652 bp	0.5	58.6
ycf2-ndhB_p2	GTCTTGTACAGGCGGCAATA	CTGCTTCAGCTTTAGCCACT	50.0	50.0	58.0	58.2	2088 bp	0.5	58.4
ycf2-ndhB_p3	GTCTTGTACAGGCGGCAATA	TCAGTCTACGTACGGGAACA	50.0	50.0	58.0	57.8	1797 bp	0.5	58.4
ycf2-ndhB_p4	GTCTTGTACAGGCGGCAATA	TGTTCAGTCTACGTACGGGA	50.0	50.0	58.0	57.8	1800 bp	0.5	58.4
ycf2-ndhB_p5	ACAGGCGGCAATATCTTTGT	ATTCTGGTGTGGATGGCAAG	45.0	50.0	57.9	58.2	1645 bp	0.5	57.7