Hotspot

ycf1

SSC · 120373-125808 · 5436 bp

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Pi 0.0509

MarkerSeek score 73.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5436
variable_sites: 501
pi: 0.0509
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGCTATCCCAAGCTTCTGC	ATGGAATCGACCATTTCGCT	50.0	45.0	58.0	57.7	1308-1326 bp	1.0	86.3
ycf1_p2	ACCATTCGGGAAGTTGCAAT	ATGGAATCGACCATTTCGCT	45.0	45.0	58.1	57.7	1439-1457 bp	1.0	85.7
ycf1_p3	ATCCTCAGACCATTCGGGAA	ATGGAATCGACCATTTCGCT	50.0	45.0	58.1	57.7	1453-1465 bp	0.75	72.2
ycf1_p4	TCAGTATTCGCCTTGTCAGC	ATGGAATCGACCATTTCGCT	50.0	45.0	58.0	57.7	933 bp	0.5	57.3
ycf1_p5	AGTATTCGCCTTGTCAGCAG	ATGGAATCGACCATTTCGCT	50.0	45.0	58.0	57.7	931 bp	0.5	57.2