Hotspot

rps15-ycf1

SSC · 120024-120372 · 349 bp

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Pi 0.0969

MarkerSeek score 66.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 349
variable_sites: 60
pi: 0.0969
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GACAAATAGGCCAGGAGTCG	GGGTATATTGCCTGCTGCTT	55.0	50.0	58.1	58.0	1135-1170 bp	0.75	78.3
rps15-ycf1_p2	AACACCATGCATTGATGGGT	GGGTATATTGCCTGCTGCTT	45.0	50.0	58.1	58.0	1385-1420 bp	0.75	77.3
rps15-ycf1_p3	AACCGAAGAACACCATGCAT	GGGTATATTGCCTGCTGCTT	45.0	50.0	58.1	58.0	1393-1428 bp	0.75	77.0
rps15-ycf1_p4	ACCGAAGAACACCATGCATT	GGGTATATTGCCTGCTGCTT	45.0	50.0	58.1	58.0	1392-1427 bp	0.75	77.0
rps15-ycf1_p5	TATTGCCTAGTTGTTCGGGC	GGGTATATTGCCTGCTGCTT	50.0	50.0	58.0	58.0	1584-1619 bp	0.75	76.7