Hotspot

psbE-petL

LSC · 62942-63764 · 823 bp

Back to Callitriche

Pi 0.0494

MarkerSeek score 72.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 72
pi: 0.0494
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAGCCACTAGTAGAAAACCA	50.0	40.0	57.8	53.9	893-949 bp	1.0	52.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCAA	50.0	40.0	57.8	53.9	892-948 bp	1.0	52.3
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AAGCCACTAGTAGAAAACCA	55.0	40.0	58.2	53.9	888-944 bp	1.0	52.0
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGCCACTAGTAGAAAACCAA	55.0	40.0	58.2	53.9	887-943 bp	1.0	52.0
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCAAA	50.0	38.1	57.8	54.8	892-948 bp	1.0	51.4