Hotspot

ycf4-cemA

LSC · 58757-59601 · 845 bp

Back to Callitriche

Pi 0.0665

MarkerSeek score 72.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 94
pi: 0.0665
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGGTGCACAATTTCCTGGAA	ATTAGTTCCCAACCGTGTGG	45.0	50.0	57.9	57.8	1288-1691 bp	1.0	88.1
ycf4-cemA_p2	TATTGCGTTGACTCGGACTG	ATTAGTTCCCAACCGTGTGG	50.0	50.0	58.0	57.8	1466 bp	0.5	58.4
ycf4-cemA_p3	TATTGCGTTGACTCGGACTG	TTCCTGCGCCCAAGAATTAA	50.0	45.0	58.0	57.8	1368 bp	0.5	58.3
ycf4-cemA_p4	TATTGCGTTGACTCGGACTG	CCTGCGCCCAAGAATTAAGA	50.0	50.0	58.0	58.2	1366 bp	0.5	57.9
ycf4-cemA_p5	TATTGCGTTGACTCGGACTG	TCCTGCGCCCAAGAATTAAG	50.0	50.0	58.0	58.2	1367 bp	0.5	57.9