Hotspot

rpoC2-rpoC1

LSC · 19985-20150 · 166 bp

Back to Callitriche

Pi 0.175

MarkerSeek score 75.50

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 56
pi: 0.175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	TGAAATACCAGATTGGGCCG	GGGGTTTTCTCAAGCCTGTT	50.0	50.0	57.9	58.3	212-229 bp	1.0	89.2
rpoC2-rpoC1_p2	AATACCAGATTGGGCCGTTC	GGGGTTTTCTCAAGCCTGTT	50.0	50.0	57.9	58.3	209-226 bp	1.0	89.2
rpoC2-rpoC1_p3	GAAATACCAGATTGGGCCGT	GGGGTTTTCTCAAGCCTGTT	50.0	50.0	57.9	58.3	211-228 bp	1.0	89.2
rpoC2-rpoC1_p4	TGAAATACCAGATTGGGCCG	TACAGGGGTTTTCTCAAGCC	50.0	50.0	57.9	57.4	216-233 bp	1.0	87.0
rpoC2-rpoC1_p5	AATACCAGATTGGGCCGTTC	TACAGGGGTTTTCTCAAGCC	50.0	50.0	57.9	57.4	213-230 bp	1.0	86.9