Hotspot

psbI-trnS-GCU

LSC · 7830-7963 · 134 bp

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Pi 0.0784
MarkerSeek score 84.60
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
134
variable_sites
18
pi
0.0784
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 TCCTATCTAATGATCCAGGACGT TGGACTAAAGCGTCGGATTG 43.5 50.0 58.0 58.0 242-251 bp 1.0 64.7
psbI-trnS-GCU_p2 TCCTATCTAATGATCCAGGACGT GTGGACTAAAGCGTCGGATT 43.5 50.0 58.0 58.0 243-252 bp 1.0 64.6
psbI-trnS-GCU_p3 TCCTATCTAATGATCCAGGACGT ATTGGGAGAGATGGCTGAGT 43.5 50.0 58.0 58.1 261-270 bp 1.0 63.2
psbI-trnS-GCU_p4 TCCTATCTAATGATCCAGGACGT GACTAAAGCGTCGGATTGCT 43.5 50.0 58.0 58.4 240-249 bp 1.0 61.9
psbI-trnS-GCU_p5 TCCTATCTAATGATCCAGGACGT CTGAGTGGACTAAAGCGTCG 43.5 55.0 58.0 58.4 247-256 bp 1.0 61.5