Hotspot

trnK-UUU-rps16

LSC · 4186-5025 · 840 bp

Back to Callitriche

Pi 0.0625
MarkerSeek score 72.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
840
variable_sites
97
pi
0.0625
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.87
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 1071-1207 bp 0.75 78.5
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1128-1266 bp 0.75 78.4
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1127-1265 bp 0.75 78.4
trnK-UUU-rps16_p4 CCCTTTCAGGATCAGTCGTG ACCCAAGAACAAGGAAACCC 55.0 50.0 58.0 57.9 1610 bp 0.5 59.2
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG ACCCAAGAACAAGGAAACCC 55.0 50.0 58.0 57.9 1609 bp 0.5 59.2