Hotspot

ycf1

SSC · 132140-137605 · 5466 bp

Back to Callisia

Pi 0.0632

MarkerSeek score 77.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5466
variable_sites: 500
pi: 0.0632
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAGGGCGTTCTGTTTCCAT	GGGTTATTCGCTGGGAGAAG	45.0	55.0	58.0	58.0	996-1020 bp	0.666667	75.9
ycf1_p2	AAGGGCGTTCTGTTTCCATT	GGGTTATTCGCTGGGAGAAG	45.0	55.0	58.0	58.0	995-1019 bp	0.666667	75.9
ycf1_p3	TGATTATGCCTCGGCGAAAA	GGGTTATTCGCTGGGAGAAG	45.0	55.0	58.0	58.0	359 bp	0.666667	72.1
ycf1_p4	TTGATTATGCCTCGGCGAAA	GGGTTATTCGCTGGGAGAAG	45.0	55.0	58.0	58.0	360 bp	0.666667	72.1
ycf1_p5	CGGATTGGTGTGGATAACGT	GGGTTATTCGCTGGGAGAAG	50.0	55.0	58.0	58.0	338 bp	0.333333	52.8