Hotspot

clpP1

LSC · 75119-77876 · 2758 bp

Back to Callisia

Pi 0.0514

MarkerSeek score 75.30

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2758
variable_sites: 174
pi: 0.0514
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TCTGATTGGCTGTCTTGTGT	AATCAATTGCCACGTTTCCA	45.0	40.0	57.1	56.5	2890-2988 bp	0.666667	54.9
clpP1_p2	TCTGATTGGCTGTCTTGTGT	ATCAATTGCCACGTTTCCAT	45.0	40.0	57.1	56.3	2889-2987 bp	0.666667	53.1
clpP1_p3	TCTGATTGGCTGTCTTGTGT	AAATCAATTGCCACGTTTCCA	45.0	38.1	57.1	57.3	2891-2989 bp	0.666667	52.9
clpP1_p4	TCTGATTGGCTGTCTTGTGTT	AATCAATTGCCACGTTTCCA	42.9	40.0	57.8	56.5	2890-2988 bp	0.666667	52.7
clpP1_p5	TTCTGATTGGCTGTCTTGTGT	AATCAATTGCCACGTTTCCA	42.9	40.0	57.8	56.5	2891-2989 bp	0.666667	52.7