Hotspot

rrn5-rrn4.5

IRa · 139030-139253 · 224 bp

Back to Callisia

Pi 0.0685

MarkerSeek score 86.50

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 224
variable_sites: 23
pi: 0.0685
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	339-340 bp	1.0	84.8
rrn5-rrn4.5_p2	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	337-338 bp	1.0	84.8
rrn5-rrn4.5_p3	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	341-342 bp	1.0	84.7
rrn5-rrn4.5_p4	AGTATCGTCACCGCAGTAGA	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	339-340 bp	1.0	84.7
rrn5-rrn4.5_p5	TCCCCTACAGTATCGTCACC	CAGCTGAGGCATCCTAACAG	55.0	55.0	57.9	58.0	349-350 bp	1.0	84.4