Hotspot

accD-psaI

LSC · 63150-64256 · 1107 bp

Back to Callisia

Pi 0.152

MarkerSeek score 76.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1107
variable_sites: 167
pi: 0.152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCTACAACTGGCGGAGTAAC	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.0	58.0	1482-1876 bp	1.0	91.6
accD-psaI_p2	GTGGTTATTGTGTGCGCTTC	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1620-2014 bp	1.0	91.4
accD-psaI_p3	CCTACAACTGGCGGAGTAAC	TGGAAATTCCAACCACCAGG	55.0	50.0	58.0	58.0	1942 bp	0.333333	53.2
accD-psaI_p4	GTGGTTATTGTGTGCGCTTC	TGGAAATTCCAACCACCAGG	50.0	50.0	58.0	58.0	2080 bp	0.333333	53.0
accD-psaI_p5	CAGTACCAGACGGTTCACAG	TGGAAATTCCAACCACCAGG	55.0	50.0	57.9	58.0	1824 bp	0.333333	52.7