Hotspot

ndhC-trnV-UAC

LSC · 53931-55791 · 1861 bp

Back to Callisia

Pi 0.0765

MarkerSeek score 66.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1861
variable_sites: 94
pi: 0.0765
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.50
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CAAGCATCCCCCATAGGTTC	GTTTACACGTGCGCCAATAC	55.0	50.0	58.0	58.1	1656-2645 bp	1.0	87.2
ndhC-trnV-UAC_p2	AGCTTCTCTGGTCCTTCACT	GTTTACACGTGCGCCAATAC	50.0	50.0	58.0	58.1	1614-2603 bp	1.0	87.2
ndhC-trnV-UAC_p3	AAGCATCCCCCATAGGTTCT	GTTTACACGTGCGCCAATAC	50.0	50.0	58.1	58.1	1655-2644 bp	1.0	86.6
ndhC-trnV-UAC_p4	CAAGCATCCCCCATAGGTTC	TACACGTGCGCCAATACTTT	55.0	45.0	58.0	58.2	1653-2642 bp	1.0	86.6
ndhC-trnV-UAC_p5	CAAGCATCCCCCATAGGTTC	TTACACGTGCGCCAATACTT	55.0	45.0	58.0	58.2	1654-2643 bp	1.0	86.6