Hotspot

trnF-GAA-ndhJ

LSC · 51825-52271 · 447 bp

Back to Callisia

Pi 0.0544

MarkerSeek score 84.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 35
pi: 0.0544
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AAAGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	600-627 bp	1.0	86.5
trnF-GAA-ndhJ_p2	AAGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	599-626 bp	1.0	86.5
trnF-GAA-ndhJ_p3	TCGTGTCACCAGTTCAAGTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	555-582 bp	1.0	86.2
trnF-GAA-ndhJ_p4	CGTGTCACCAGTTCAAGTCT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	554-581 bp	1.0	86.1
trnF-GAA-ndhJ_p5	CTCGTGTCACCAGTTCAAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	556-583 bp	1.0	86.1