Hotspot

rps4-trnT-UGU

LSC · 49072-49634 · 563 bp

Back to Callisia

Pi 0.074

MarkerSeek score 85.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 563
variable_sites: 62
pi: 0.074
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	675-790 bp	1.0	83.8
rps4-trnT-UGU_p2	CTCCCAGACGGCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	58.3	58.2	644-759 bp	1.0	83.8
rps4-trnT-UGU_p3	CTCCCAGACGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.3	57.5	683-798 bp	1.0	80.7
rps4-trnT-UGU_p4	TAGTCCTGGTAAAGCTCCCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.4	57.9	689-804 bp	1.0	80.7
rps4-trnT-UGU_p5	CTCCCAGACGGCGTATTTTT	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.3	57.5	673-788 bp	1.0	80.6