Hotspot

trnE-UUC-trnT-GGU

LSC · 33530-34724 · 1195 bp

Back to Callisia

Pi 0.113

MarkerSeek score 85.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1195
variable_sites: 161
pi: 0.113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CTTCTTTTCGTGGTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.2	57.9	1115-1301 bp	1.0	89.7
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.3	57.9	1077-1263 bp	1.0	89.1
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.7	57.9	1056-1242 bp	1.0	88.9
trnE-UUC-trnT-GGU_p4	CTTCTTTTCGTGGTACCCCC	ACGACTTACGCCTTACCATG	55.0	50.0	58.2	57.7	1144-1330 bp	1.0	88.2
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	ACGACTTACGCCTTACCATG	55.0	50.0	58.3	57.7	1106-1292 bp	1.0	87.6