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psbM-trnD-GUC

LSC · 31794-32842 · 1049 bp

Back to Callisia

Pi 0.0479

MarkerSeek score 83.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 69
pi: 0.0479
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGACTGACTGTTTTTACGTAGA	TTCAATTGGTCAGAGCACCG	36.4	50.0	55.1	58.5	1145-1212 bp	1.0	48.6
psbM-trnD-GUC_p2	TTGACTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	35.0	50.0	53.0	58.5	1146-1213 bp	1.0	48.6
psbM-trnD-GUC_p3	TGACTGACTGTTTTTACGTAGATG	TTCAATTGGTCAGAGCACCG	37.5	50.0	57.0	58.5	1145-1212 bp	1.0	48.6
psbM-trnD-GUC_p4	TTTGACTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	33.3	50.0	53.9	58.5	1147-1214 bp	1.0	48.6
psbM-trnD-GUC_p5	TCATTTTGACTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	32.0	50.0	57.9	58.5	1151-1218 bp	1.0	48.6