Hotspot

rpoB-trnC-GCA

LSC · 28844-30445 · 1602 bp

Back to Callisia

Pi 0.0616

MarkerSeek score 83.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1602
variable_sites: 137
pi: 0.0616
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGGATCCCCACTTACACAAG	TTACCACTTGGCTATGTCGC	55.0	50.0	58.0	58.0	2337-2440 bp	1.0	88.5
rpoB-trnC-GCA_p2	CGGATCCCCACTTACACAAG	TGTTTGTTGATCAGGCGACA	55.0	45.0	58.0	58.0	2399-2502 bp	1.0	88.1
rpoB-trnC-GCA_p3	TGAAATTCTTCCCCCAAGCC	TACCCCTAGAGTCCACTCCT	50.0	55.0	58.1	58.0	2314-2792 bp	0.666667	74.1
rpoB-trnC-GCA_p4	CGGATCCCCACTTACACAAG	TACCCCTAGAGTCCACTCCT	55.0	55.0	58.0	58.0	2918 bp	0.333333	53.1
rpoB-trnC-GCA_p5	CGGATCCCCACTTACACAAG	AAAAAGCTTGGCAAAACGCT	55.0	40.0	58.0	58.0	2995 bp	0.333333	53.0