Hotspot

ndhF

SSC · 117314-119557 · 2244 bp

Back to Calligonum

Pi 0.00199

MarkerSeek score 43.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 34
pi: 0.00199
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACTAGATCAAAATACCTCGCA	GAAAGAGGTAAGAGCCGGTG	38.1	55.0	54.5	58.0	2316 bp	1.0	44.9
ndhF_p2	ACTAGATCAAAATACCTCGCA	AGAGGTAAGAGCCGGTGAAT	38.1	50.0	54.5	58.1	2313 bp	1.0	43.9
ndhF_p3	ACTAGATCAAAATACCTCGCA	AAGAGCCGGTGAATCGAAAA	38.1	45.0	54.5	57.8	2307 bp	1.0	43.5
ndhF_p4	ACTAGATCAAAATACCTCGCA	GAGGTAAGAGCCGGTGAATC	38.1	55.0	54.5	57.8	2312 bp	1.0	43.2
ndhF_p5	ACTAGATCAAAATACCTCGCA	AGCCGGTGAATCGAAAATCA	38.1	45.0	54.5	57.9	2304 bp	0.952381	42.1