Hotspot

ndhA

SSC · 126886-129149 · 2264 bp

Back to Calligonum

Pi 0.00199

MarkerSeek score 52.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2264
variable_sites: 33
pi: 0.00199
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTTTCTTTCTCTTGTTTGGGACA	CCCGCCGGGTTTTATCAATT	39.1	50.0	57.4	58.9	2439-2495 bp	1.0	46.3
ndhA_p2	TGTTTCTTTCTCTTGTTTGGGAC	CCCGCCGGGTTTTATCAATT	39.1	50.0	57.4	58.9	2440-2496 bp	1.0	46.3
ndhA_p3	TCTTTCTCTTGTTTGGGACA	CCCGCCGGGTTTTATCAATT	40.0	50.0	54.4	58.9	2436-2492 bp	1.0	46.2
ndhA_p4	GTTTCTTTCTCTTGTTTGGGACA	CCGCCGGGTTTTATCAATTT	39.1	45.0	57.4	57.0	2438-2494 bp	1.0	45.9
ndhA_p5	TGTTTCTTTCTCTTGTTTGGGAC	CCGCCGGGTTTTATCAATTT	39.1	45.0	57.4	57.0	2439-2495 bp	1.0	45.9