Hotspot

clpP

LSC · 72499-74904 · 2406 bp

Back to Calligonum

Pi 0.00101

MarkerSeek score 52.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2406
variable_sites: 18
pi: 0.00101
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCAATCCTAACCTGGTGAGT	AGCCCCATTATTACGTTTCCA	45.0	42.9	55.5	57.3	2506-2576 bp	1.0	49.6
clpP_p2	GATCAATCCTAACCTGGTGAGT	AGCCCCATTATTACGTTTCCA	45.5	42.9	57.2	57.3	2508-2578 bp	1.0	47.9
clpP_p3	AGGGACTGGTTTAGATCAATCC	AGCCCCATTATTACGTTTCCA	45.5	42.9	57.2	57.3	2521-2591 bp	1.0	47.7
clpP_p4	GGGACTGGTTTAGATCAATCCT	AGCCCCATTATTACGTTTCCA	45.5	42.9	57.2	57.3	2520-2590 bp	1.0	47.7
clpP_p5	ATCAATCCTAACCTGGTGAGT	AGCCCCATTATTACGTTTCCA	42.9	42.9	56.0	57.3	2507-2577 bp	1.0	46.3