Hotspot

rpl32-trnL-UAG

SSC · 120715-121338 · 624 bp

Back to Calligonum

Pi 0.00252
MarkerSeek score 56.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
624
variable_sites
13
pi
0.00252
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCGAAATCTCTTTCTACCGG TCGAACCGAGATGCTTTAGC 47.6 50.0 57.8 58.1 751-766 bp 1.0 72.9
rpl32-trnL-UAG_p2 AGCGAAATCTCTTTCTACCGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.8 57.9 725-740 bp 1.0 72.6
rpl32-trnL-UAG_p3 AGCGAAATCTCTTTCTACCGG AGCAGCGTGTCTACCAATTT 47.6 45.0 57.8 57.8 718-733 bp 1.0 72.2
rpl32-trnL-UAG_p4 AGCGAAATCTCTTTCTACCGG CTAAGAGCAGCGTGTCTACC 47.6 55.0 57.8 57.8 723-738 bp 1.0 72.0
rpl32-trnL-UAG_p5 AGAAAAGGATATTCGGCAGCA TCGAACCGAGATGCTTTAGC 42.9 50.0 57.7 58.1 789-804 bp 1.0 71.8