Hotspot

trnR-ACG-trnN-GUU

IRb · 110466-111076 · 611 bp

Back to Calligonum

Pi 0.00338

MarkerSeek score 51.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 611
variable_sites: 9
pi: 0.00338
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	707-721 bp	1.0	79.1
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	694-708 bp	1.0	79.1
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	695-709 bp	1.0	78.7
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CGTTCCTCAGTAGCTCAGTG	55.0	55.0	57.8	57.7	739-753 bp	1.0	78.0
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	55.0	50.0	57.8	57.7	737-751 bp	1.0	77.7