Hotspot

psbT-psbN

LSC · 77153-77223 · 71 bp

Back to Calligonum

Pi 0.0241

MarkerSeek score 56.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 6
pi: 0.0241
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GAGATCCGTTCGAGGAACAC	50.0	55.0	57.6	58.1	134 bp	1.0	80.0
psbT-psbN_p2	TCGAGAACCGCCTAAAGTTC	AGAGATCCGTTCGAGGAACA	50.0	50.0	57.6	58.2	135 bp	1.0	79.4
psbT-psbN_p3	GAGAACCGCCTAAAGTTCCA	GAGATCCGTTCGAGGAACAC	50.0	55.0	57.5	58.1	132 bp	1.0	79.2
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	ACCCTAGTCGCCATCTTTCT	50.0	50.0	57.6	58.1	225 bp	1.0	78.6
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	ACCCTAGTCGCCATCTTTCT	50.0	50.0	57.5	58.1	223 bp	1.0	77.7