Hotspot

psbB-psbT

LSC · 76891-77050 · 160 bp

Back to Calligonum

Pi 0.000595

MarkerSeek score 29.00

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 1
pi: 0.000595
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCCAACTACAAGAAGACAAGCA	GAACTTTAGGCGGTTCTCGA	40.9	50.0	57.6	57.6	271-276 bp	1.0	59.8
psbB-psbT_p2	TCCAACTACAAGAAGACAAGCA	TGGAACTTTAGGCGGTTCTC	40.9	50.0	57.6	57.5	273-278 bp	1.0	58.9
psbB-psbT_p3	TCCAACTACAAGAAGACAAGCA	GGAACTTTAGGCGGTTCTCG	40.9	55.0	57.6	58.6	272-277 bp	1.0	57.6
psbB-psbT_p4	TCCAACTACAAGAAGACAAGCA	AGTTGGAACTTTAGGCGGTT	40.9	45.0	57.6	57.3	276-281 bp	1.0	57.5
psbB-psbT_p5	CCAACTACAAGAAGACAAGCAG	GAACTTTAGGCGGTTCTCGA	45.5	50.0	57.3	57.6	270-275 bp	1.0	57.3