Hotspot

trnF-GAA-ndhJ

LSC · 50719-51466 · 748 bp

Back to Calligonum

Pi 0.00266

MarkerSeek score 56.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 16
pi: 0.00266
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GATAATCATCCGCGCCTGAA	50.0	50.0	58.1	58.2	863-910 bp	1.0	83.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAATCATCCGCGCCTGAAA	50.0	45.0	58.1	57.7	862-909 bp	1.0	82.9
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTG	50.0	50.0	58.1	58.3	865-912 bp	1.0	82.7
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	833-880 bp	1.0	82.3
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	GATAATCATCCGCGCCTGAA	50.0	50.0	58.2	58.2	905-952 bp	1.0	81.8