Hotspot

trnT-UGU-trnL-UAA

LSC · 48698-49673 · 976 bp

Back to Calligonum

Pi 0.00504

MarkerSeek score 61.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 976
variable_sites: 26
pi: 0.00504
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	1004-1049 bp	1.0	83.0
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	1035-1080 bp	1.0	82.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	998-1043 bp	1.0	81.7
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	CGTCTACCGATTTCGCCATA	55.0	50.0	58.2	57.6	1029-1074 bp	1.0	81.4
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.5	58.2	996-1041 bp	1.0	80.1