Hotspot

pafI-trnS-GGA

LSC · 45869-46786 · 918 bp

Back to Calligonum

Pi 0.00247
MarkerSeek score 53.30
Primer available yes
Label pafI-trnS-GGA

Feature Metadata

label_name
pafI-trnS-GGA
feature_type
igs
region
LSC
length_bp
918
variable_sites
18
pi
0.00247
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-GGA_p1 TTCGCCTGAAGTTGTTGGAA TGTTGGTGAGTTGGGTGAAG 45.0 50.0 58.0 57.9 1114-1126 bp 1.0 82.0
pafI-trnS-GGA_p2 TTTCGCCTGAAGTTGTTGGA TGTTGGTGAGTTGGGTGAAG 45.0 50.0 58.0 57.9 1115-1127 bp 1.0 82.0
pafI-trnS-GGA_p3 CCTCCTTTTCGCCTGAAGTT TGTTGGTGAGTTGGGTGAAG 50.0 50.0 58.1 57.9 1121-1133 bp 1.0 81.5
pafI-trnS-GGA_p4 TTGGCCACAACTGAAAAGGT TGTTGGTGAGTTGGGTGAAG 45.0 50.0 58.1 57.9 1077-1089 bp 1.0 81.3
pafI-trnS-GGA_p5 TTCGCCTGAAGTTGTTGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 58.0 58.2 1092-1104 bp 1.0 81.3