Hotspot

rpoB-trnC-GCA

LSC · 23938-28200 · 4263 bp

Back to Calligonum

Pi 0.00138

MarkerSeek score 48.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 4263
variable_sites: 44
pi: 0.00138
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GCGGGGGTCTATCCAAATTT	TCCCTAATGACATAAGAAAGTCAA	50.0	33.3	57.9	55.5	304-332 bp	1.0	44.9
rpoB-trnC-GCA_p2	GCGGGGGTCTATCCAAATTT	TCCCTAATGACATAAGAAAGTCAAA	50.0	32.0	57.9	56.2	304-332 bp	1.0	44.9
rpoB-trnC-GCA_p3	GCGGGGGTCTATCCAAATTT	TGTTTCCCTAATGACATAAGAAAGT	50.0	32.0	57.9	56.4	308-336 bp	1.0	44.8
rpoB-trnC-GCA_p4	GCGGGGGTCTATCCAAATTT	TGTTTCCCTAATGACATAAGAAAGTC	50.0	34.6	57.9	57.4	308-336 bp	1.0	44.8
rpoB-trnC-GCA_p5	GCGGGGGTCTATCCAAATTT	TTCCCTAATGACATAAGAAAGTCAA	50.0	32.0	57.9	56.2	305-333 bp	1.0	44.8