Hotspot

trnS-GCU-trnG-UCC

LSC · 8209-9294 · 1086 bp

Back to Calligonum

Pi 0.00275

MarkerSeek score 59.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1086
variable_sites: 25
pi: 0.00275
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	1192-1247 bp	1.0	55.9
trnS-GCU-trnG-UCC_p2	CGAATGACTCGTACAACGGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.7	55.5	1162-1217 bp	1.0	55.2
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	1194-1249 bp	1.0	54.8
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	AGGAATCAAATTGAAGGATCCCT	50.0	39.1	58.2	57.5	1228-1269 bp	0.952381	53.9
trnS-GCU-trnG-UCC_p5	CGAATGACTCGTACAACGGA	AGGAATCAAATTGAAGGATCCCT	50.0	39.1	57.7	57.5	1198-1239 bp	0.952381	53.3