Hotspot

ndhA

SSC · 120125-122309 · 2185 bp

Back to Callicarpa

Pi 0.00248

MarkerSeek score 51.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2185
variable_sites: 26
pi: 0.00248
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTGAGTTAGCATAGGGAACA	ACTTGCAAATTCTTCCGCAG	42.9	45.0	56.1	57.3	2376-2385 bp	1.0	52.1
ndhA_p2	ACTGAGTTAGCATAGGGAACA	CTTGCAAATTCTTCCGCAGT	42.9	45.0	56.1	57.3	2375-2384 bp	1.0	52.1
ndhA_p3	TGTTGACCAGAGTTCAAGAAC	CTTGCAAATTCTTCCGCAGT	42.9	45.0	56.0	57.3	2394-2403 bp	1.0	51.9
ndhA_p4	TGTTTGTTGACCAGAGTTCA	ACTTGCAAATTCTTCCGCAG	40.0	45.0	55.0	57.3	2399-2408 bp	0.95	49.6
ndhA_p5	TGTTTGTTGACCAGAGTTCA	CTTGCAAATTCTTCCGCAGT	40.0	45.0	55.0	57.3	2398-2407 bp	0.95	49.6