Hotspot

rpl32-trnL

SSC · 113834-114638 · 805 bp

Back to Callicarpa

Pi 0.00638

MarkerSeek score 66.90

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 805
variable_sites: 26
pi: 0.00638
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGGCAGCGTTAAAAGCATTT	TTTAGGAGATGACATGCCGC	45.0	50.0	57.9	58.0	976-1000 bp	1.0	84.8
rpl32-trnL_p2	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	921-945 bp	1.0	84.4
rpl32-trnL_p3	GGGCAGCGTTAAAAGCATTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.9	57.8	914-938 bp	1.0	84.0
rpl32-trnL_p4	GGGCAGCGTTAAAAGCATTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.9	57.8	920-944 bp	1.0	83.9
rpl32-trnL_p5	GGGCAGCGTTAAAAGCATTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.9	57.8	919-943 bp	1.0	83.9