Hotspot

trnN-GUU-ndhF

IRb · 109490-110893 · 1404 bp

Back to Callicarpa

Pi 0.000364

MarkerSeek score 50.50

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1404
variable_sites: 5
pi: 0.000364
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GGGTGGCCGTATTTCTTCTT	50.0	50.0	58.2	57.9	1436-1576 bp	1.0	82.1
trnN-GUU-ndhF_p2	CCCCAAGTAGGATTCGAACC	GGGTGGCCGTATTTCTTCTT	55.0	50.0	57.7	57.9	1444-1584 bp	1.0	80.7
trnN-GUU-ndhF_p3	GGATTCGAACCTACGACCAA	GGGTGGCCGTATTTCTTCTT	50.0	50.0	57.3	57.9	1435-1575 bp	1.0	78.0
trnN-GUU-ndhF_p4	TCTCCCCAAGTAGGATTCGA	GGGTGGCCGTATTTCTTCTT	50.0	50.0	57.2	57.9	1447-1587 bp	1.0	76.6
trnN-GUU-ndhF_p5	TAGGATTCGAACCTACGACCA	GGGTGGCCGTATTTCTTCTT	47.6	50.0	58.0	57.9	1437-1577 bp	1.0	75.3