Hotspot

rbcL-accD

LSC · 57850-58483 · 634 bp

Back to Callicarpa

Pi 0.00385
MarkerSeek score 43.10
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
634
variable_sites
13
pi
0.00385
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 CAGTGGATACTTTGGATCCGG ACCTGCGTTCTAACTCCTTC 52.4 50.0 58.2 57.3 750-778 bp 0.95 66.6
rbcL-accD_p2 AGTGGATACTTTGGATCCGGA ACCTGCGTTCTAACTCCTTC 47.6 50.0 58.2 57.3 749-777 bp 0.95 66.0
rbcL-accD_p3 GTGGATACTTTGGATCCGGA ACCTGCGTTCTAACTCCTTC 50.0 50.0 56.7 57.3 748-776 bp 0.95 65.6
rbcL-accD_p4 AGTGGATACTTTGGATCCGG ACCTGCGTTCTAACTCCTTC 50.0 50.0 56.7 57.3 749-777 bp 0.95 65.5
rbcL-accD_p5 CAGTGGATACTTTGGATCCGG TCCCATACCTGCGTTCTAAC 52.4 50.0 58.2 57.0 756-784 bp 0.95 64.8