Hotspot

rps16-trnQ-UUG

LSC · 6493-7617 · 1125 bp

Back to Callicarpa

Pi 0.00404
MarkerSeek score 55.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1125
variable_sites
24
pi
0.00404
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAAATTGAAATGGGGCGTGG 50.0 50.0 57.9 58.0 1273-1305 bp 1.0 81.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1256-1288 bp 1.0 81.2
rps16-trnQ-UUG_p3 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1215-1247 bp 1.0 81.1
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1198-1230 bp 1.0 80.7
rps16-trnQ-UUG_p5 CAACGGATCGTGTCCTTCAA GAAATTGAAATGGGGCGTGG 50.0 50.0 58.2 58.0 1274-1306 bp 1.0 80.6