Hotspot

matK-rps16

LSC · 3734-5358 · 1625 bp

Back to Callicarpa

Pi 0.00275

MarkerSeek score 61.10

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1625
variable_sites: 30
pi: 0.00275
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GCACGATTATGAGCAAATGCA	GTGCTCAACCCACAGGAAC	42.9	57.9	57.9	58.7	1748-1821 bp	1.0	63.5
matK-rps16_p2	GCACGATTATGAGCAAATGCA	TGCTCAACCCACAGGAACT	42.9	52.6	57.9	58.8	1747-1820 bp	1.0	62.7
matK-rps16_p3	AGGAAGTCGTGTTGTTGAGA	GTGCTCAACCCACAGGAAC	45.0	57.9	56.7	58.7	1697-1770 bp	1.0	61.9
matK-rps16_p4	AGCACGATTATGAGCAAATGC	GTGCTCAACCCACAGGAAC	42.9	57.9	57.7	58.7	1749-1822 bp	1.0	61.3
matK-rps16_p5	AGGAAGTCGTGTTGTTGAGA	TGCTCAACCCACAGGAACT	45.0	52.6	56.7	58.8	1696-1769 bp	1.0	61.1