Hotspot

rps12-clpP

LSC · 72410-72571 · 162 bp

Back to Callianthemum

Pi 0.0248

MarkerSeek score 64.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 4
pi: 0.0248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CGAAGAGCAGGGGATTTTGT	TGTTGATCTTGTAGCGGTCG	50.0	50.0	58.2	58.0	278-279 bp	1.0	80.0
rps12-clpP_p2	CGAAGAGCAGGGGATTTTGT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.2	57.9	311-312 bp	1.0	79.0
rps12-clpP_p3	CGAAGAGCAGGGGATTTTGT	GTTGATCTTGTAGCGGTCGA	50.0	50.0	58.2	57.7	277-278 bp	1.0	77.9
rps12-clpP_p4	CGAAGAGCAGGGGATTTTGT	TCCGAAGACATGGAAAGGGA	50.0	50.0	58.2	58.3	337-338 bp	1.0	77.0
rps12-clpP_p5	CGAAGAGCAGGGGATTTTGT	TCGAAAACACCGGGGATTTC	50.0	50.0	58.2	58.5	261-262 bp	1.0	76.3