Hotspot

trnP-UGG-psaJ

LSC · 69251-69680 · 430 bp

Back to Callianthemum

Pi 0.0186

MarkerSeek score 55.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 430
variable_sites: 8
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	519-605 bp	1.0	75.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	518-604 bp	1.0	75.5
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	530-616 bp	1.0	75.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	529-615 bp	1.0	75.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTGCCACGGAGAGATATGTT	50.0	50.0	58.1	57.4	532-618 bp	1.0	75.4