Hotspot

petL-petG

LSC · 68492-68671 · 180 bp

Back to Callianthemum

Pi 0.0278

MarkerSeek score 68.50

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 5
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TTCTGCTGGCAGCTTTAACT	TCAAAGGTCCAACTGATCGC	45.0	50.0	57.7	58.2	362-363 bp	1.0	77.4
petL-petG_p2	TATTTCGGGTTTCTGCTGGC	TCAAAGGTCCAACTGATCGC	50.0	50.0	58.5	58.2	372-373 bp	1.0	75.2
petL-petG_p3	GGTTTCTGCTGGCAGCTTTA	TCAAAGGTCCAACTGATCGC	50.0	50.0	58.8	58.2	365-366 bp	1.0	73.5
petL-petG_p4	TTCTGCTGGCAGCTTTAACT	AGGCCTAACACGATTCCAAA	45.0	45.0	57.7	57.1	286-287 bp	1.0	72.2
petL-petG_p5	TTTCTGCTGGCAGCTTTAAC	TCAAAGGTCCAACTGATCGC	45.0	50.0	56.9	58.2	363-364 bp	1.0	70.9