Hotspot

rpoB-trnC-GCA

LSC · 26330-27575 · 1246 bp

Back to Callianthemum

Pi 0.012

MarkerSeek score 65.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 15
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1334-1362 bp	1.0	74.9
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCG	CAAGCAAGGGGATTTCGAGT	50.0	50.0	57.3	58.2	1375-1403 bp	1.0	74.2
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCG	ATCAGCAAGCAAGGGGATTT	50.0	45.0	57.3	57.8	1380-1408 bp	1.0	73.6
rpoB-trnC-GCA_p4	ATTCCCTCATTTCCATCCCG	TTCATTGATCAGGCGACACC	50.0	50.0	57.3	58.3	1353-1381 bp	1.0	73.5
rpoB-trnC-GCA_p5	ATTCCCTCATTTCCATCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.3	58.4	1316-1344 bp	1.0	72.7