Hotspot

trnR-UCU-atpA

LSC · 9615-9741 · 127 bp

Back to Callianthemum

Pi 0.0556

MarkerSeek score 77.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 7
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAAGTCGGAATGAAAGGCGT	AGAAGCGCAAGTCCTTTTGA	45.0	45.0	58.1	58.0	287-288 bp	1.0	81.1
trnR-UCU-atpA_p2	AAAGTCGGAATGAAAGGCGT	CAGGAACAGATGGAGCGATT	45.0	50.0	58.1	57.7	256-257 bp	1.0	79.1
trnR-UCU-atpA_p3	AAAGTCGGAATGAAAGGCGT	AGACATTCACCGAAGAAGCG	45.0	50.0	58.1	58.3	300-301 bp	1.0	79.0
trnR-UCU-atpA_p4	AAAGTCGGAATGAAAGGCGT	ATTCAGGAACAGATGGAGCG	45.0	50.0	58.1	57.7	259-260 bp	1.0	79.0
trnR-UCU-atpA_p5	ATGAAAGGCGTCCATTGTCT	AGAAGCGCAAGTCCTTTTGA	45.0	45.0	57.5	58.0	278-279 bp	1.0	78.1