Hotspot

trnS-GCU-trnG-UCC

LSC · 7946-8599 · 654 bp

Back to Callianthemum

Pi 0.0153
MarkerSeek score 67.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
654
variable_sites
10
pi
0.0153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 761-857 bp 1.0 68.1
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 760-856 bp 1.0 68.0
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA AACCGAAAGACCCCTTAACT 50.0 45.0 58.2 56.4 815-911 bp 1.0 66.8
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 762-858 bp 1.0 66.2
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 761-857 bp 1.0 66.2