Hotspot

trnH-GUG-psbA

LSC · 78-278 · 201 bp

Back to Callianthemum

Pi 0.0588

MarkerSeek score 79.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 11
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	318-333 bp	1.0	84.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	332-347 bp	1.0	84.6
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	331-346 bp	1.0	84.6
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	333-348 bp	1.0	84.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TATCATCAACCGCGCTAACC	55.0	50.0	58.1	58.1	329-344 bp	1.0	83.8