Hotspot

ycf4

Genome · 63114-63752 · 639 bp

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Pi 0

MarkerSeek score 32.90

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: Genome
length_bp: 639
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ATTTCCGGCAATTGCAATGG	TTGTAAACGTGTTTCCGGGT	45.0	45.0	58.0	58.0	1439 bp	1.0	83.7
ycf4_p2	GAGGGCGAAATTCATCCAGT	TTGTAAACGTGTTTCCGGGT	50.0	45.0	58.0	58.0	1895-1900 bp	1.0	83.5
ycf4_p3	ATTTCCGGCAATTGCAATGG	CGTGTTTCCGGGTATTCCTT	45.0	50.0	58.0	57.9	1432 bp	1.0	83.1
ycf4_p4	GGCAATTGCAATGGCTTCTT	TTGTAAACGTGTTTCCGGGT	45.0	45.0	57.9	58.0	1433 bp	1.0	83.1
ycf4_p5	GAGGGCGAAATTCATCCAGT	CGTGTTTCCGGGTATTCCTT	50.0	50.0	58.0	57.9	1888-1893 bp	1.0	83.0