Hotspot

ycf1

Genome · 111143-116389 · 5247 bp

Back to Callerya

Pi 0.000889
MarkerSeek score 53.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
Genome
length_bp
5247
variable_sites
7
pi
0.000889
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CAAGAACTGGTCATTCCCCC AACAAATGCCATCCCAGGTT 55.0 45.0 58.2 58.0 318 bp 1.0 79.5
ycf1_p2 CAAGAACTGGTCATTCCCCC ACAAATGCCATCCCAGGTTT 55.0 45.0 58.2 58.0 317 bp 1.0 79.5
ycf1_p3 CCAAGAACTGGTCATTCCCC AACAAATGCCATCCCAGGTT 55.0 45.0 58.2 58.0 319 bp 1.0 79.5
ycf1_p4 CCAAGAACTGGTCATTCCCC ACAAATGCCATCCCAGGTTT 55.0 45.0 58.2 58.0 318 bp 1.0 79.5
ycf1_p5 CAAGAACTGGTCATTCCCCC AAATGCCATCCCAGGTTTCT 55.0 45.0 58.2 57.4 315 bp 1.0 74.7