Hotspot

ndhB-rps7

Genome · 98972-99338 · 367 bp

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Pi 0.00545

MarkerSeek score 43.60

Primer available yes

Label ndhB-rps7

Feature Metadata

label_name: ndhB-rps7
feature_type: igs
region: Genome
length_bp: 367
variable_sites: 3
pi: 0.00545
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-rps7_p1	CTCCATCGGAACAATAGGGC	ATGGCAGAGGCAAATAGAGC	55.0	50.0	58.1	58.0	591 bp	1.0	79.6
ndhB-rps7_p2	CTCCATCGGAACAATAGGGC	CTGTGGAAAGCCGTATTCGA	55.0	50.0	58.1	58.0	1177 bp	1.0	79.4
ndhB-rps7_p3	CTCCATCGGAACAATAGGGC	TCTGTGGAAAGCCGTATTCG	55.0	50.0	58.1	58.0	1178 bp	1.0	79.4
ndhB-rps7_p4	CTCCATCGGAACAATAGGGC	ACGGATTCTCCCTTTTACGC	55.0	50.0	58.1	58.0	1042 bp	1.0	79.4
ndhB-rps7_p5	TCGGGAGTCCATTGATGAGA	CTGTGGAAAGCCGTATTCGA	50.0	50.0	57.8	58.0	1702 bp	1.0	79.0